LOCUS       40924_29776        7538 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pmAlbEPintPlauGH DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M, 
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Establishment of a humanized model of liver using NOD/Shi-scid 
            IL2Rgnull mice
  JOURNAL   Biochem. Biophys. Res. Commun. 377 (1), 248-252 (2008)
  PUBMED    18840406
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Taniguchi K, Monnai M, Wakui M, 
            Suematsu M, Ito M, Peltz G, Nakamura M.
  TITLE     Expression vector pmAlbEPintPlauGH DNA, complete sequence
  JOURNAL   Published Only in Database (2008)
REFERENCE   3  (bases 1 to 7538)
  AUTHORS   Suemizu H, Hasegawa M, Kawai K, Nakamura M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute 
            for Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   4  (bases 1 to 7538)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "377"; issue: "1"; 
            pages: "248-252"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2008)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-AUG-2008) Contact:Hiroshi Suemizu Central Institute for 
            Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        678..3013
                     /label=Alb
                     /note="Mouse albumin promoter"
     intron          3130..3262
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        3307..3325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3340..4638
                     /gene="Plau"
                     /label=Plau
                     /note="Urokinase-type plasminogen activator from Mus
                     musculus. Accession#: P06869"
     polyA_signal    4694..5316
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        complement(5350..5368)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5389..5405)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5413..5429)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5437..5467)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5482..5503)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5791..6379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6553..7410)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7411..7515)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gcttccttag catgacgttc cacttttttc taaggtggag
      721 cttacttctt tgatttgatc ttttgtgaaa cttttggaaa ttacccatct tcctaagctt
      781 ctgcttctct cagttttctg cttgctcatt ccacttttcc agctgaccct gccccctacc
      841 aacattgctc cacaagcaca aattcatcca gagaaaataa attctaagtt ttatagttgt
      901 ttggatcgca taggtagcta aagaggtggc aacccacaca tccttaggca tgagcttgat
      961 tttttttgat ttagaacctt cccctctctg ttcctagact acactacaca ttctgcaagc
     1021 atagcacaga gcaatgttct actttaatta ctttcatttt cttgtatcct cacagcctag
     1081 aaaataacct gcgttacagc atccactcag tatcccttga gcatgaggtg acactactta
     1141 acatagggac gagatggtac tttgtgtctc ctgctctgtc agcagggcac tgtacttgct
     1201 gataccaggg aatgtttgtt cttaaatacc atcattccgg acgtgtttgc cttggccagt
     1261 tttccatgta catgcagaaa gaagtttgga ctgatcaata cagtcctctg cctttaaagc
     1321 aataggaaaa ggccaacttg tctacgttta gtatgtggct gtagaaaggg tatagatata
     1381 aaaattaaaa ctaatgaaat ggcagtctta cacatttttg gcagcttatt taaagtcttg
     1441 gtgttaagta cgctggagct gtcacagcta ccaatcaggc atgtctggga atgagtacac
     1501 ggggaccata agttactgac attcgtttcc cattccattt gaatacacac ttttgtcatg
     1561 gtattgcttg ctgaaattgt tttgcaaaaa aaaccccttc aaattcatat atattatttt
     1621 aataaatgaa ttttaattta tctcaatgtt ataaaaaagt caattttaat aattaggtac
     1681 ttatataccc aataatatct aacaatcatt tttaaacatt tgtttattga gcttattatg
     1741 gatgaatcta tctctatata ctctatatac tctaaaaaag aagaaagacc atagacaatc
     1801 atctatttga tatgtgtaaa gtttacatgt gagtagacat cagatgctcc atttctcact
     1861 gtaataccat ttatagttac ttgcaaaact aactggaatt ctaggactta aatattttaa
     1921 gttttagctg ggtgactggt tggaaaattt taggtaagta ctgaaaccaa gagattataa
     1981 aacaataaat tctaaagttt tagaagtgat cataatcaaa tattaccctc taatgaaaat
     2041 attccaaagt tgagctacag aaatttcaac ataagataat tttagctgta acaatgtaat
     2101 ttgttgtcta ttttcttttg agatacagtt ttttctgtct agctttggct gtcctggacc
     2161 ttgctctgta gaccaggttg gtcttgaact cagagatctg cttgcctctg ccttgcaagt
     2221 gctaggatta aaagcatgtg ccaccactgc ctggctacaa tctatgtttt ataagagatt
     2281 ataaagctct ggctttgtga cattaatctt tcagataata agtcttttgg attgtgtctg
     2341 gagaacatac agactgtgag cagatgttca gaggtatatt tgcttagggg tgaattcaat
     2401 ctgcagcaat aattatgagc agaattactg acacttccat tttatacatt ctacttgctg
     2461 atctatgaaa catagataag catgcaggca ttcatcatag ttttctttat ctggaaaaac
     2521 attaaatatg aaagaagcac tttattaata cagtttagat gtgttttgcc atcttttaat
     2581 ttcttaagaa atactaagct gatgcagagt gaagagtgtg tgaaaagcag tggtgcagct
     2641 tggcttgaac tcgttctcca gcttgggatc gacctgcagg catgcttcca tgccaaggcc
     2701 cacactgaaa tgctcaaatg ggagacaaag agattaagct cttatgtaaa atttgctgtt
     2761 ttacataact ttaatgaatg gacaaagtct tgtgcatggg ggtgggggtg gggttagagg
     2821 ggaacagctc cagatggcaa acatacgcaa gggatttagt caaacaactt tttggcaaag
     2881 atggtatgat tttgtaatgg ggtaggaacc aatgaaatgc gaggtaagta tggttaatga
     2941 tctacagtta ttggttaaag aagtatatta gagcgagtct ttctgcacac agatcacctt
     3001 tcctatcaac cccgggatcc aagctttatt gcggtagttt atcacagtta aattgctaac
     3061 gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc
     3121 actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
     3181 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
     3241 cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag
     3301 agtacttaat acgactcact ataggctagc ggcactacca tgaaagtctg gctggcgagc
     3361 ctgttcctct gcgccttggt ggtgaaaaac tctgaaggtg gcagtgtact tggagctcct
     3421 gatgaatcaa actgtggctg tcagaacgga ggtgtatgcg tgtcctacaa gtacttctcc
     3481 agaattcgcc gatgcagctg cccaaggaaa ttccaggggg agcactgtga gatagatgca
     3541 tcaaaaacct gctatcatgg aaatggtgac tcttaccgag gaaaggccaa cactgatacc
     3601 aaaggtcggc cctgcctggc ctggaatgcg cctgctgtcc ttcagaaacc ctacaatgcc
     3661 cacagacctg atgctattag cctaggcctg gggaaacaca attactgcag gaaccctgac
     3721 aaccagaagc gaccctggtg ctatgtgcag attggcctaa ggcagtttgt ccaagaatgc
     3781 atggtgcatg actgctctct tagcaaaaag ccttcttcgt ctgtagacca acaaggcttc
     3841 cagtgtggcc agaaggctct aaggccccgc tttaagattg ttgggggaga attcactgag
     3901 gtggagaacc agccctggtt cgcagccatc taccagaaga acaagggagg aagtcctccc
     3961 tcctttaaat gtggtgggag tctcatcagt ccttgctggg tggccagtgc cgcacactgc
     4021 ttcattcaac tcccaaagaa ggaaaactac gttgtctacc tgggtcagtc gaaggagagc
     4081 tcctataatc ctggagagat gaagtttgag gtggagcagc tcatcttgca cgaatactac
     4141 agggaagaca gcctggccta ccataatgat attgccttgc tgaagatacg taccagcacg
     4201 ggccaatgtg cacagccatc caggtccata cagaccatct gcctgccccc aaggtttact
     4261 gatgctccgt ttggttcaga ctgtgagatc actggctttg gaaaagagtc tgaaagtgac
     4321 tatctctatc caaagaacct gaaaatgtcc gtcgtaaagc ttgtttctca tgaacagtgt
     4381 atgcagcccc actactatgg ctctgaaatt aattataaaa tgctgtgtgc tgcggaccca
     4441 gagtggaaaa cagattcctg caagggcgat tctggaggac cgcttatctg taacatcgaa
     4501 ggccgcccaa ctctgagtgg gattgtgagc tggggccgag gatgtgcaga gaaaaacaag
     4561 cccggtgtct acacgagggt ctcacacttc ctggactgga ttcaatccca cattggagaa
     4621 gagaaaggtc tggccttctg atggccctca ggtagctgag ggaagaaaca gatgggtcac
     4681 ttgttgtcga cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
     4741 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
     4801 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
     4861 agttgggaag acaacctgta gggcctgcgg ggtctattcg ggaaccaagc tggagtgcag
     4921 tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc
     4981 agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt
     5041 tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg
     5101 tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct
     5161 tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag acacagcata
     5221 ggctacctgc catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc
     5281 atgcgttggg tccactcagt agatgcctgt tgaattctcg agggggggcc cggtacccag
     5341 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     5401 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     5461 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     5521 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5581 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5641 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5701 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6421 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6481 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6541 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6601 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6661 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6721 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6781 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6841 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6901 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6961 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7021 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7081 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7141 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7201 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7261 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7321 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7381 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7501 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
